Coding

Part:BBa_K2587005

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-15)


lasR

LasR is the regulator protein of the quorum sensing system of Pseudomonas aeruginosa. In this case the sequence is codon optimized for E. coli and together with LasI and Plas induces expression of a downstream gene in a population density dependent manner.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 579
    Illegal SpeI site found at 738
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 731


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Categories
Parameters
None